OrangerV1, France, Analysis, bibRecord, 000245

DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus

Identifieur interne : 000245 ( France/Analysis ); précédent : 000244; suivant : 000246

DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus

Auteurs : F. Luro [France] ; F. Laigret [France] ; J.-M. Bove [France] ; P. Ollitrault [France]

Source :

HortScience [ 0018-5345 ] ; 1995.

RBID : Pascal:95-0500201

Descripteurs français

Pascal (Inist)
- Etude en laboratoire, Variabilité génétique, Séquence répétée, Méthode fingerprint, Amplification génique, Réaction chaîne polymérase, Cultivar, Citrus volkameriana, Citrus sinensis, Citrus reticulata, DNA, Diversité génétique.

English descriptors

KwdEn :
- Citrus reticulata, Citrus sinensis, Citrus volkameriana, Cultivar, DNA, Fingerprint method, Gene amplification, Genetic diversity, Genetic variability, Laboratory study, Polymerase chain reaction, Repeated sequence.

Abstract

We used three short repetitive nucleotide sequences [(GTG)5, (TCC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted citrus DNA or as single primers in polymerase chain reaction amplification experiments with total genomic DNA. We tested the ability of the sequences to discriminate between seedlings of zygotic or nucellar origin in the progeny of a Volkamer lemon (Citrus volkameriana Ten. & Pasq.) tree. The genetic variability within two species [Citrus sinensis (L.) Osbeck (sweet oranges) and Citrus reticulata Blanco and relatives (mandarins)] also was evaluated. DNA amplified fingerprinting with single primers was the more successful technique for discriminating between nucellar and zygotic seedlings. Although we were not able to distinguish among 10 cultivars of C. sinensis, all 10 C. reticulata cultivars tested were distinguishable. However, it still is difficult to identify the putative parents of a hybrid plant when the two parental genomes are closely related.

Affiliations:

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to stream Main, to step Curation: 003645
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Pascal:95-0500201

Le document en format XML

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<front><div type="abstract" xml:lang="en">We used three short repetitive nucleotide sequences [(GTG)5, (TCC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted citrus DNA or as single primers in polymerase chain reaction amplification experiments with total genomic DNA. We tested the ability of the sequences to discriminate between seedlings of zygotic or nucellar origin in the progeny of a Volkamer lemon (Citrus volkameriana Ten. & Pasq.) tree. The genetic variability within two species [Citrus sinensis (L.) Osbeck (sweet oranges) and Citrus reticulata Blanco and relatives (mandarins)] also was evaluated. DNA amplified fingerprinting with single primers was the more successful technique for discriminating between nucellar and zygotic seedlings. Although we were not able to distinguish among 10 cultivars of C. sinensis, all 10 C. reticulata cultivars tested were distinguishable. However, it still is difficult to identify the putative parents of a hybrid plant when the two parental genomes are closely related.</div>
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DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus

DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri